PrimePCR™ Probe Assay: NUDT12, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031455
Assay Design:   Intron-spanning
Chromosome Location:   5:102890493-102891676question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000230792 ENST00000507423

Gene Information

Nucleotides are involved in numerous biochemical reactions and pathways within the cell as substrates cofactors and effectors. Nudix hydrolases such as NUDT12 regulate the concentrations of individual nucleotides and of nucleotide ratios in response to changing circumstances (Abdelraheim et al. 2003 [PubMed 12790796]).[supplied by OMIM Mar 2008]

Gene Symbol:   NUDT12
Gene Name:   nudix (nucleoside diphosphate linked moiety X)-type motif 12
Aliases:   DKFZp761I172
RefSeq:   NC_000005.9 NT_034772.6
Ensembl:   ENSG00000112874
Entrez:   83594
UniGene:   Hs.434289
Chromosome Mapping:   5q21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.030000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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