PrimePCR™ Probe Assay: GRAP2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: GRAP2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GRAP2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031435
Assay Design:   Intron-spanning
Chromosome Location:   22:40365453-40366969question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000344138 ENST00000407075 ENST00000544006 ENST00000540310 ENST00000543252 ENST00000399090 ENST00000544756

Gene Information

This gene encodes a member of the GRB2/Sem5/Drk family. This member is an adaptor-like protein involved in leukocyte-specific protein-tyrosine kinase signaling. Like its related family member GRB2-related adaptor protein (GRAP) this protein contains an SH2 domain flanked by two SH3 domains. This protein interacts with other proteins such as GRB2-associated binding protein 1 (GAB1) and the SLP-76 leukocyte protein (LCP2) through its SH3 domains. Transcript variants utilizing alternative polyA sites exist. [provided by RefSeq Jul 2008]

Gene Symbol:   GRAP2
Gene Name:   GRB2-related adaptor protein 2
Aliases:   GADS, GRAP-2, GRB2L, GRBLG, GRID, GRPL, GrbX, Grf40, Mona, P38
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000100351
Entrez:   9402
Chromosome Mapping:   22q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.640000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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