This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: GRAP2, Human
PrimePCR™ Template for Probe Assay: GRAP2, Human
This gene encodes a member of the GRB2/Sem5/Drk family. This member is an adaptor-like protein involved in leukocyte-specific protein-tyrosine kinase signaling. Like its related family member GRB2-related adaptor protein (GRAP) this protein contains an SH2 domain flanked by two SH3 domains. This protein interacts with other proteins such as GRB2-associated binding protein 1 (GAB1) and the SLP-76 leukocyte protein (LCP2) through its SH3 domains. Transcript variants utilizing alternative polyA sites exist. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.