PrimePCR™ Probe Assay: ATP7A, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031403
Assay Design:   Intron-spanning
Chromosome Location:   X:77166294-77227217question
Amplicon Length:   109
Splice Variants Targeted:   ENST00000341514 ENST00000343912 ENST00000343533 ENST00000350425 ENST00000400860

Gene Information

This gene encodes a transmembrane protein that functions in copper transport across membranes. This protein is localized to the trans-Golgi network where it is predicted to supply copper to copper-dependent enzymes in the secretory pathway. It relocalizes to the plasma membrane under conditions of elevated extracellular copper and functions in the efflux of copper from cells. Mutations in this gene are associated with Menkes disease X-linked cutis laxa and occipital horn syndrome. [provided by RefSeq Oct 2011]

Gene Symbol:   ATP7A
Gene Name:   ATPase, Cu++ transporting, alpha polypeptide
Aliases:   DSMAX, FLJ17790, MK, MNK, SMAX3
RefSeq:   NG_021360.1 NC_000023.10 NG_013224.1 NT_011651.17
Ensembl:   ENSG00000165240
Entrez:   538
UniGene:   Hs.496414
Chromosome Mapping:   Xq21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.220000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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