This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: ABHD12, Human
PrimePCR™ Template for Probe Assay: ABHD12, Human
This gene encodes an enzyme that catalyzes the hydrolysis of 2-arachidonoyl glycerol (2-AG) the main endocannabinoid lipid transmitter that acts on cannabinoid receptors CB1 and CB2. The endocannabinoid system is involved in a wide range of physiological processes including neurotransmission mood appetite pain appreciation addiction behavior and inflammation. Mutations in this gene are associated with the neurodegenerative disease PHARC (polyneuropathy hearing loss ataxia retinitis pigmentosa and cataract) resulting from an inborn error of endocannabinoid metabolism. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq Jan 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.