PrimePCR™ Probe Assay: ABHD12, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031387
Assay Design:   Intron-spanning
Chromosome Location:   20:25300878-25304045question
Amplicon Length:   132
Splice Variants Targeted:   ENST00000376542 ENST00000339157 ENST00000450393 ENST00000526543

Gene Information

This gene encodes an enzyme that catalyzes the hydrolysis of 2-arachidonoyl glycerol (2-AG) the main endocannabinoid lipid transmitter that acts on cannabinoid receptors CB1 and CB2. The endocannabinoid system is involved in a wide range of physiological processes including neurotransmission mood appetite pain appreciation addiction behavior and inflammation. Mutations in this gene are associated with the neurodegenerative disease PHARC (polyneuropathy hearing loss ataxia retinitis pigmentosa and cataract) resulting from an inborn error of endocannabinoid metabolism. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq Jan 2011]

Gene Symbol:   ABHD12
Gene Name:   abhydrolase domain containing 12
Aliases:   ABHD12A, BEM46L2, C20orf22, DKFZp434P106, PHARC, dJ965G21.2
RefSeq:   NC_000020.10 NT_011387.8 NG_028119.1
Ensembl:   ENSG00000100997
Entrez:   26090
UniGene:   Hs.441550
Chromosome Mapping:   20p11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 34.210000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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