This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: CBLN1, Human
PrimePCR™ Template for Probe Assay: CBLN1, Human
This gene encodes a cerebellum-specific precursor protein precerebellin with similarity to the globular (non-collagen-like) domain of complement component C1qB. Precerebellin is processed to give rise to several derivatives including the hexadecapeptide cerebellin which is highly enriched in postsynaptic structures of Purkinje cells. Cerebellin has also been found in human and rat adrenals where it has been shown to enhance the secretory activity of this gland. [provided by RefSeq Aug 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.