PrimePCR™ Probe Assay: CBLN1, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031341
Assay Design:   Intron-spanning
Chromosome Location:   16:49313389-49314873question
Amplicon Length:   134
Splice Variants Targeted:   ENST00000219197 ENST00000536749

Gene Information

This gene encodes a cerebellum-specific precursor protein precerebellin with similarity to the globular (non-collagen-like) domain of complement component C1qB. Precerebellin is processed to give rise to several derivatives including the hexadecapeptide cerebellin which is highly enriched in postsynaptic structures of Purkinje cells. Cerebellin has also been found in human and rat adrenals where it has been shown to enhance the secretory activity of this gland. [provided by RefSeq Aug 2008]

Gene Symbol:   CBLN1
Gene Name:   cerebellin 1 precursor
Aliases:   Not Available
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000102924
Entrez:   869
UniGene:   Hs.458423
Chromosome Mapping:   16q12.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.480000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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