PrimePCR™ Probe Assay: KLHDC3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: KLHDC3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: KLHDC3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031333
Assay Design:   Intron-spanning
Chromosome Location:   6:42987032-42988459question
Amplicon Length:   98
Splice Variants Targeted:   ENST00000326974 ENST00000432243 ENST00000426116 ENST00000394096 ENST00000332245 ENST00000244670

Gene Information

The protein encoded by this gene contains six repeated kelch motifs that are structurally similar to recombination activating gene 2 (RAG2) a protein involved in the activation of the V(D)J recombination. In mouse this gene is found to express specifically in testis. Its expression in pachytene spermatocytes is localized to cytoplasma and meiotic chromatin which suggests that this gene may be involved in meiotic recombination. Alternatively spliced transcript variants have been found. [provided by RefSeq Jun 2011]

Gene Symbol:   KLHDC3
Gene Name:   kelch domain containing 3
Aliases:   PEAS, RP1-20C7.3, dJ20C7.3, hPEAS
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000124702
Entrez:   116138
Chromosome Mapping:   6p21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 34.850000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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