PrimePCR™ Probe Assay: IL13RA2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: IL13RA2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: IL13RA2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031128
Assay Design:   Intron-spanning
Chromosome Location:   X:114250328-114251859question
Amplicon Length:   148
Splice Variants Targeted:   ENST00000371936 ENST00000243213

Gene Information

The protein encoded by this gene is closely related to Il13RA1 a subuint of the interleukin 13 receptor complex. This protein binds IL13 with high affinity but lacks cytoplasmic domain and does not appear to function as a signal mediator. It is reported to play a role in the internalization of IL13. [provided by RefSeq Jul 2008]

Gene Symbol:   IL13RA2
Gene Name:   interleukin 13 receptor, alpha 2
Aliases:   CD213A2, CT19, IL-13R, IL13BP
RefSeq:   NC_000023.10 NG_012514.1 NT_028405.12
Ensembl:   ENSG00000123496
Entrez:   3598
Chromosome Mapping:   Xq13.1-q28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.950000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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