PrimePCR™ Probe Assay: MYLK3, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031113
Assay Design:   Intron-spanning
Chromosome Location:   16:46764563-46766204question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000394809 ENST00000536476

Gene Information

Phosphorylation of cardiac myosin heavy chains (see MYH7B MIM 609928) and light chains (see MYL2 MIM 160781) by a kinase such as MYLK3 potentiates the force and rate of cross-bridge recruitment in cardiac myocytes (Chan et al. 2008 [PubMed 18202317]).[supplied by OMIM Jul 2008]

Gene Symbol:   MYLK3
Gene Name:   myosin light chain kinase 3
Aliases:   MGC126319, MGC126320, MLCK, MLCK2, caMLCK
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000140795
Entrez:   91807
UniGene:   Hs.130465
Chromosome Mapping:   16q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.070000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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