This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: MYLK3, Human
PrimePCR™ Template for Probe Assay: MYLK3, Human
Phosphorylation of cardiac myosin heavy chains (see MYH7B MIM 609928) and light chains (see MYL2 MIM 160781) by a kinase such as MYLK3 potentiates the force and rate of cross-bridge recruitment in cardiac myocytes (Chan et al. 2008 [PubMed 18202317]).[supplied by OMIM Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.