PrimePCR™ Probe Assay: SLC5A11, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SLC5A11, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SLC5A11, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031098
Assay Design:   Intron-spanning
Chromosome Location:   16:24920290-24921662question
Amplicon Length:   134
Splice Variants Targeted:   ENST00000347898 ENST00000424767 ENST00000545376 ENST00000449109 ENST00000539472

Gene Information

Cotransporters such as SLC5A11 represent a major class of proteins that make use of ion gradients to drive active transport for the cellular accumulation of nutrients neurotransmitters osmolytes and ions Roll et al. (2002) [PubMed 12039040].[supplied by OMIM Mar 2008]

Gene Symbol:   SLC5A11
Gene Name:   solute carrier family 5 (sodium/glucose cotransporter), member 11
Aliases:   KST1, RKST1, SGLT6, SMIT2
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000158865
Entrez:   115584
Chromosome Mapping:   16pter-p11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 34.350000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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