This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: TGFB1, Human
PrimePCR™ Template for Probe Assay: TGFB1, Human
This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines which are multifunctional peptides that regulate proliferation differentiation adhesion migration and other functions in many cell types. Many cells have TGFB receptors and the protein positively and negatively regulates many other growth factors. The secreted protein is cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide and is found in either a latent form composed of a TGFB1 homodimer a LAP homodimer and a latent TGFB1-binding protein or in an active form composed of a TGFB1 homodimer. The mature peptide may also form heterodimers with other TGFB family members. This gene is frequently upregulated in tumor cells and mutations in this gene result in Camurati-Engelmann disease.[provided by RefSeq Oct 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.