PrimePCR™ Probe Assay: TGFB1, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030973
Assay Design:   Intron-spanning
Chromosome Location:   19:41850663-41854265question
Amplicon Length:   143
Splice Variants Targeted:   ENST00000221930

Gene Information

This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines which are multifunctional peptides that regulate proliferation differentiation adhesion migration and other functions in many cell types. Many cells have TGFB receptors and the protein positively and negatively regulates many other growth factors. The secreted protein is cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide and is found in either a latent form composed of a TGFB1 homodimer a LAP homodimer and a latent TGFB1-binding protein or in an active form composed of a TGFB1 homodimer. The mature peptide may also form heterodimers with other TGFB family members. This gene is frequently upregulated in tumor cells and mutations in this gene result in Camurati-Engelmann disease.[provided by RefSeq Oct 2009]

Gene Symbol:   TGFB1
Gene Name:   transforming growth factor, beta 1
Aliases:   CED, DPD1, LAP, TGFB, TGFbeta
RefSeq:   NC_000019.9 NG_013091.1 NG_013364.1 NT_011109.16
Ensembl:   ENSG00000105329
Entrez:   7040
UniGene:   Hs.645227
Chromosome Mapping:   19q13.2,19q13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.020000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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