This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: CTSK, Human
PrimePCR™ Template for Probe Assay: CTSK, Human
The protein encoded by this gene is a lysosomal cysteine proteinase involved in bone remodeling and resorption. This protein which is a member of the peptidase C1 protein family is predominantly expressed in osteoclasts. However the encoded protein is also expressed in a significant fraction of human breast cancers where it could contribute to tumor invasiveness. Mutations in this gene are the cause of pycnodysostosis an autosomal recessive disease characterized by osteosclerosis and short stature. This gene may be subject to RNA editing. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.