PrimePCR™ Probe Assay: SUPT7L, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: SUPT7L, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SUPT7L, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030905
Assay Design:   Intron-spanning
Chromosome Location:   2:27878363-27880263question
Amplicon Length:   129
Splice Variants Targeted:   ENST00000337768 ENST00000406540 ENST00000405491 ENST00000464789 ENST00000404798

Gene Information

SUPT7L is a protein subunit of the human STAGA complex (SPT3; (MIM 602947)/TAF9 (MIM 600822)/GCN5 (MIM 602301) acetyltransferase complex) which is a chromatin-modifying multiprotein complex (Martinez et al. 2001 [PubMed 11564863]).[supplied by OMIM Apr 2009]

Gene Symbol:   SUPT7L
Gene Name:   suppressor of Ty 7 (S. cerevisiae)-like
Aliases:   KIAA0764, MGC90306, SPT7L, STAF65, STAF65(gamma), STAF65G, SUPT7H
RefSeq:   NC_000002.11 NT_022184.15
Ensembl:   ENSG00000119760
Entrez:   9913
Chromosome Mapping:   2p23.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 34.650000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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