PrimePCR™ Probe Assay: SHPK, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030735
Assay Design:   Intron-spanning
Chromosome Location:   17:3514220-3518756question
Amplicon Length:   143
Splice Variants Targeted:   ENST00000225519

Gene Information

The protein encoded by this gene has weak homology to several carbohydrate kinases a class of proteins involved in the phosphorylation of sugars as they enter a cell inhibiting return across the cell membrane. Sequence variation between this novel gene and known carbohydrate kinases suggests the possibility of a different substrate cofactor or changes in kinetic properties distinguishing it from other carbohydrate kinases. The gene resides in a region commonly deleted in cystinosis patients suggesting a role as a modifier for the cystinosis phenotype. The genomic region is also rich in Alu repetitive sequences frequently involved in chromosomal rearrangements. [provided by RefSeq Jul 2008]

Gene Symbol:   SHPK
Gene Name:   sedoheptulokinase
Aliases:   CARKL, FLJ32478, SHK
RefSeq:   NC_000017.10 NG_012489.1 NT_010718.16
Ensembl:   ENSG00000197417
Entrez:   23729
UniGene:   Hs.579217
Chromosome Mapping:   17p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996500
y-intercept 36.220000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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