PrimePCR™ Probe Assay: TMEM181, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030329
Assay Design:   Intron-spanning
Chromosome Location:   6:159006388-159010811question
Amplicon Length:   148
Splice Variants Targeted:   ENST00000367090 ENST00000314630

Gene Information

The TMEM181 gene encodes a putative G protein-coupled receptor expressed on the cell surface (Carette et al. 2009 [PubMed 19965467]; Wollscheid et al. 2009 [PubMed 19349973]).[supplied by OMIM Jan 2010]

Gene Symbol:   TMEM181
Gene Name:   transmembrane protein 181
Aliases:   GPR178, KIAA1423
RefSeq:   NC_000006.11 NT_025741.15
Ensembl:   ENSG00000146433
Entrez:   57583
UniGene:   Hs.99145
Chromosome Mapping:   6q25.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.110000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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