PrimePCR™ Probe Assay: PHIP, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PHIP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PHIP, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030328
Assay Design:   Intron-spanning
Chromosome Location:   6:79651016-79655101question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000275034 ENST00000355098

Gene Information

PHIP binds the pleckstrin homology (PH) domain of insulin receptor substrate-1 (IRS1; MIM 147545) modulates insulin signaling and plays a role in pancreatic beta cell growth and survival (Farhang-Fallah et al. 2000 [PubMed 11018022]; Podcheko et al. 2007 [PubMed 17636024]).[supplied by OMIM Jun 2009]

Gene Symbol:   PHIP
Gene Name:   pleckstrin homology domain interacting protein
Aliases:   DCAF14, FLJ20705, FLJ45918, MGC90216, WDR11, ndrp
RefSeq:   NC_000006.11 NT_007299.13
Ensembl:   ENSG00000146247
Entrez:   55023
Chromosome Mapping:   6q14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.070000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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