PrimePCR™ Probe Assay: PRPF6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PRPF6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PRPF6, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030327
Assay Design:   Intron-spanning
Chromosome Location:   20:62612575-62614479question
Amplicon Length:   145
Splice Variants Targeted:   ENST00000266079 ENST00000535781

Gene Information

The protein encoded by this gene appears to be involved in pre-mRNA splicing possibly acting as a bridging factor between U5 and U4/U6 snRNPs in formation of the spliceosome. The encoded protein also can bind androgen receptor providing a link between transcriptional activation and splicing. [provided by RefSeq Jul 2008]

Gene Symbol:   PRPF6
Gene Name:   PRP6 pre-mRNA processing factor 6 homolog (S. cerevisiae)
Aliases:   ANT-1, C20orf14, Prp6, TOM, U5-102K, hPrp6
RefSeq:   NC_000020.10 NT_011333.6
Ensembl:   ENSG00000101161
Entrez:   24148
Chromosome Mapping:   20q13.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 34.900000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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