PrimePCR™ Probe Assay: RBP3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030296
Assay Design:   Intron-spanning
Chromosome Location:   10:48383909-48385906question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000224600

Gene Information

Interphotoreceptor retinol-binding protein is a large glycoprotein known to bind retinoids and found primarily in the interphotoreceptor matrix of the retina between the retinal pigment epithelium and the photoreceptor cells. It is thought to transport retinoids between the retinal pigment epithelium and the photoreceptors a critical role in the visual process.The human IRBP gene is approximately 9.5 kbp in length and consists of four exons separated by three introns. The introns are 1.6-1.9 kbp long. The gene is transcribed by photoreceptor and retinoblastoma cells into an approximately 4.3-kilobase mRNA that is translated and processed into a glycosylated protein of 135000 Da. The amino acid sequence of human IRBP can be divided into four contiguous homology domains with 33-38% identity suggesting a series of gene duplication events. In the gene the boundaries of these domains are not defined by exon-intron junctions as might have been expected. The first three homology domains and part of the fourth are all encoded by the first large exon which is 3180 base pairs long. The remainder of the fourth domain is encoded in the last three exons which are 191 143 and approximately 740 base pairs long respectively. [provided by RefSeq Jul 2008]

Gene Symbol:   RBP3
Gene Name:   retinol binding protein 3, interstitial
Aliases:   D10S64, D10S65, D10S66, IRBP, RBPI
RefSeq:   NC_000010.10 NT_030772.10
Ensembl:   ENSG00000107618
Entrez:   5949
UniGene:   Hs.591928
Chromosome Mapping:   10q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 34.810000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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