PrimePCR™ Probe Assay: LINS, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030292
Assay Design:   Intron-spanning
Chromosome Location:   15:101110261-101112147question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000314742

Gene Information

The Drosophila segment polarity gene lin encodes a protein lines which plays important roles in development of the epidermis and hindgut. This gene encodes a protein containing a lines-like domain. This gene is located on chromosome 15 and clustered with the gene encoding ankyrin repeat and SOCS box-containing protein 7. [provided by RefSeq Sep 2010]

Gene Symbol:   LINS
Gene Name:   lines homolog (Drosophila)
Aliases:   FLJ10583, LINS1, WINS1
RefSeq:   NC_000015.9 NT_010274.17
Ensembl:   ENSG00000140471
Entrez:   55180
UniGene:   Hs.105633
Chromosome Mapping:   15q26.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.130000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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