PrimePCR™ Probe Assay: ST8SIA5, Human

print
RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030001
Assay Design:   Intron-spanning
Chromosome Location:   18:44266208-44268798question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000315087 ENST00000538168 ENST00000536490

Gene Information

The protein encoded by this gene is a type II membrane protein that may be present in the Golgi apparatus. The encoded protein which is a member of glycosyltransferase family 29 may be involved in the synthesis of gangliosides GD1c GT1a GQ1b and GT3 from GD1a GT1b GM1b and GD3 respectively. [provided by RefSeq Jul 2008]

Gene Symbol:   ST8SIA5
Gene Name:   ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 5
Aliases:   MGC119670, MGC119671, SIAT8E, ST8SiaV
RefSeq:   NC_000018.9 NT_010966.14
Ensembl:   ENSG00000101638
Entrez:   29906
UniGene:   Hs.465025
Chromosome Mapping:   18q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.030000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
Get Help

Call us at 1-800-4-BIORAD (1-800-424-6723)

Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.