This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: IDO2, Human
PrimePCR™ Template for Probe Assay: IDO2, Human
Along with the enzymes encoded by the INDO (MIM 147435) and TDO2 (MIM 191070) genes the enzyme encoded by the INDOL1 gene metabolizes tryptophan in the kynurenine pathway (Ball et al. 2007 [PubMed 17499941]).[supplied by OMIM Feb 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.