PrimePCR™ Probe Assay: PSTPIP1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PSTPIP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PSTPIP1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029963
Assay Design:   Intron-spanning
Chromosome Location:   15:77323572-77324717question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000379595 ENST00000267939

Gene Information

The protein encoded by this gene binds to the cytoplasmic tail of CD2 an effector of T cell activation and adhesion negatively affecting CD2-triggered T cell activation. The encoded protein appears to be a scaffold protein and a regulator of the actin cytoskeleton. It has also been shown to bind ABL1 PTPN18 WAS CD2AP and PTPN12. Mutations in this gene are a cause of PAPA syndrome. [provided by RefSeq Jul 2008]

Gene Symbol:   PSTPIP1
Gene Name:   proline-serine-threonine phosphatase interacting protein 1
Aliases:   CD2BP1, CD2BP1L, CD2BP1S, H-PIP, PAPAS, PSTPIP
RefSeq:   NC_000015.9 NG_007526.1 NT_010194.17
Ensembl:   ENSG00000140368
Entrez:   9051
Chromosome Mapping:   15q24-q25.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 34.240000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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