PrimePCR™ Probe Assay: GATM, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: GATM, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GATM, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029935
Assay Design:   Intron-spanning
Chromosome Location:   15:45654372-45656996question
Amplicon Length:   136
Splice Variants Targeted:   ENST00000396659

Gene Information

This gene encodes a mitochondrial enzyme that belongs to the amidinotransferase family. This enzyme is involved in creatine biosynthesis whereby it catalyzes the transfer of a guanido group from L-arginine to glycine resulting in guanidinoacetic acid the immediate precursor of creatine. Mutations in this gene cause arginine:glycine amidinotransferase deficiency an inborn error of creatine synthesis characterized by mental retardation language impairment and behavioral disorders. [provided by RefSeq Jul 2008]

Gene Symbol:   GATM
Gene Name:   glycine amidinotransferase (L-arginine:glycine amidinotransferase)
Aliases:   AGAT, AT
RefSeq:   NC_000015.9 NG_011674.1 NT_010194.17
Ensembl:   ENSG00000171766
Entrez:   2628
Chromosome Mapping:   15q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.450000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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