PrimePCR™ Probe Assay: IL31RA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: IL31RA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: IL31RA, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029794
Assay Design:   Intron-spanning
Chromosome Location:   5:55178986-55185918question
Amplicon Length:   129
Splice Variants Targeted:   ENST00000396836 ENST00000396834 ENST00000447346 ENST00000359040 ENST00000490985 ENST00000354961 ENST00000297015

Gene Information

The protein encoded by this gene belongs to the type I cytokine receptor family. This receptor with homology to gp130 is expressed on monocytes and is involved in IL-31 signaling via activation of STAT-3 and STAT-5. It functions either as a monomer or as part of a receptor complex with oncostatin M receptor (OSMR). Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq Jun 2011]

Gene Symbol:   IL31RA
Gene Name:   interleukin 31 receptor A
Aliases:   CRL, CRL3, GLM-R, GLMR, GPL, IL-31RA, MGC125346, PRO21384, hGLM-R
RefSeq:   NC_000005.9 NT_006713.15
Ensembl:   ENSG00000164509
Entrez:   133396
Chromosome Mapping:   5q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.940000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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