This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: SYTL5, Human
PrimePCR™ Template for Probe Assay: SYTL5, Human
The protein encoded by this gene belongs to the synaptotagmin-like (Slp) protein family which contains a unique homology domain at the N-terminus referred to as the Slp homology domain (SHD). The SHD functions as a binding site for Rab27A which plays a role in protein transport. Expression of this gene is restricted to placenta and liver suggesting that it might be involved in Rab27A-dependent membrane trafficking in specific tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Sep 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.