PrimePCR™ Probe Assay: ARSF, Human


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029760
Assay Design:   Intron-spanning
Chromosome Location:   X:3028205-3030261question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000359361 ENST00000537104 ENST00000381127

Gene Information

This gene is a member of the sulfatase family and more specifically the arylsulfatase subfamily. Members of the subfamily share similarity in sequence and splice sites and are clustered together on chromosome X suggesting that they are derived from recent gene duplication events. Sulfatases are essential for the correct composition of bone and cartilage matrix. The activity of this protein unlike that of arylsulfatase E is not inhibited by warfarin. Multiple alternatively spliced variants encoding the same protein have been identified.[provided by RefSeq Jan 2011]

Gene Symbol:   ARSF
Gene Name:   arylsulfatase F
Aliases:   ASF
RefSeq:   NC_000023.10 NG_012822.2 NT_167197.1
Ensembl:   ENSG00000062096
Entrez:   416
UniGene:   Hs.101674
Chromosome Mapping:   Xp22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 34.460000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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