This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: ARSF, Human
PrimePCR™ Template for Probe Assay: ARSF, Human
This gene is a member of the sulfatase family and more specifically the arylsulfatase subfamily. Members of the subfamily share similarity in sequence and splice sites and are clustered together on chromosome X suggesting that they are derived from recent gene duplication events. Sulfatases are essential for the correct composition of bone and cartilage matrix. The activity of this protein unlike that of arylsulfatase E is not inhibited by warfarin. Multiple alternatively spliced variants encoding the same protein have been identified.[provided by RefSeq Jan 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.