This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: HOOK3, Human
PrimePCR™ Template for Probe Assay: HOOK3, Human
Hook proteins are cytosolic coiled-coil proteins that contain conserved N-terminal domains which attach to microtubules and more divergent C-terminal domains which mediate binding to organelles. The Drosophila Hook protein is a component of the endocytic compartment.[supplied by OMIM Apr 2004]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.