PrimePCR™ Probe Assay: CAPZB, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: CAPZB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CAPZB, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029588
Assay Design:   Intron-spanning
Chromosome Location:   1:19684007-19712001question
Amplicon Length:   137
Splice Variants Targeted:   ENST00000264203 ENST00000375144 ENST00000375142 ENST00000433834 ENST00000413711 ENST00000457768 ENST00000401084 ENST00000375145 ENST00000264202

Gene Information

This gene encodes the beta subunit of the barbed-end actin binding protein which belongs to the F-actin capping protein family. The capping protein is a heterodimeric actin capping protein that blocks actin filament assembly and disassembly at the fast growing (barbed) filament ends and functions in regulating actin filament dynamics as well as in stabilizing actin filament lengths in muscle and nonmuscle cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found.[provided by RefSeq Apr 2011]

Gene Symbol:   CAPZB
Gene Name:   capping protein (actin filament) muscle Z-line, beta
Aliases:   CAPB, CAPPB, CAPZ, DKFZp686K0524, FLJ12274, FLJ44693, MGC104401, MGC129749, MGC129750
RefSeq:   NC_000001.10 NT_004610.19
Ensembl:   ENSG00000077549
Entrez:   832
Chromosome Mapping:   1p36.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 36.080000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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