PrimePCR™ Probe Assay: COL27A1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: COL27A1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: COL27A1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029540
Assay Design:   Intron-spanning
Chromosome Location:   9:117068909-117070026question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000356083 ENST00000357257

Gene Information

Fibrillar collagens such as COL27A1 compose one of the most ancient families of extracellular matrix molecules. They form major structural elements in extracellular matrices of cartilage skin and tendon (Boot-Handford et al. 2003 [PubMed 12766169]).[supplied by OMIM Mar 2008]

Gene Symbol:   COL27A1
Gene Name:   collagen, type XXVII, alpha 1
Aliases:   FLJ11895, KIAA1870, MGC11337
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000196739
Entrez:   85301
Chromosome Mapping:   9q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.700000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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