PrimePCR™ Probe Assay: ARSJ, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029505
Assay Design:   Intron-spanning
Chromosome Location:   4:114824792-114899663question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000315366 ENST00000541197

Gene Information

Sulfatases (EC 3.1.5.6) such as ARSJ hydrolyze sulfate esters from sulfated steroids carbohydrates proteoglycans and glycolipids. They are involved in hormone biosynthesis modulation of cell signaling and degradation of macromolecules (Sardiello et al. 2005 [PubMed 16174644]).[supplied by OMIM Mar 2008]

Gene Symbol:   ARSJ
Gene Name:   arylsulfatase family, member J
Aliases:   FLJ23548
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000180801
Entrez:   79642
UniGene:   Hs.22895
Chromosome Mapping:   4q26

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.920000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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