PrimePCR™ Probe Assay: WFIKKN1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: WFIKKN1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: WFIKKN1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029319
Assay Design:   Intron-spanning
Chromosome Location:   16:681396-682644question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000319070

Gene Information

This gene encodes a secreted multidomain protein consisting of a signal peptide a WAP domain a follistatin domain an immunoglobulin domain two tandem Kunitz domains and an NTR domain. These domains have been implicated frequently in inhibition of various types of proteases suggesting that the encoded protein may be a multivalent protease inhibitor and may control the action of multiple types of serine proteases as well as metalloproteinases. [provided by RefSeq Jul 2008]

Gene Symbol:   WFIKKN1
Gene Name:   WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 1
Aliases:   C16orf12, MGC126651, MGC126655, RJD2, WFIKKN
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000127578
Entrez:   117166
Chromosome Mapping:   16p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 36.140000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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