PrimePCR™ Probe Assay: SLC16A3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: SLC16A3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SLC16A3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029317
Assay Design:   Intron-spanning
Chromosome Location:   17:80195597-80196614question
Amplicon Length:   180
Splice Variants Targeted:   ENST00000392341 ENST00000392339

Gene Information

Lactic acid and pyruvate transport across plasma membranes is catalyzed by members of the proton-linked monocarboxylate transporter (MCT) family which has been designated solute carrier family-16. Each MCT appears to have slightly different substrate and inhibitor specificities and transport kinetics which are related to the metabolic requirements of the tissues in which it is found. The MCTs which include MCT1 (SLC16A1; MIM 600682) and MCT2 (SLC16A7; MIM 603654) are characterized by 12 predicted transmembrane domains (Price et al. 1998 [PubMed 9425115]).[supplied by OMIM Mar 2008]

Gene Symbol:   SLC16A3
Gene Name:   solute carrier family 16, member 3 (monocarboxylic acid transporter 4)
Aliases:   MCT 3, MCT 4, MCT-3, MCT-4, MCT3, MCT4, MGC138472, MGC138474
RefSeq:   NC_000017.10 NT_010663.15
Ensembl:   ENSG00000141526
Entrez:   9123
Chromosome Mapping:   17q25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.570000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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