PrimePCR™ Probe Assay: WBSCR16, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: WBSCR16, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: WBSCR16, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029265
Assay Design:   Intron-spanning
Chromosome Location:   7:74486505-74489268question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000329959 ENST00000503250 ENST00000543840

Gene Information

This gene encodes an RCC1-like G-exchanging factor. It is deleted in Williams syndrome a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. [provided by RefSeq Jul 2008]

Gene Symbol:   WBSCR16
Gene Name:   Williams-Beuren syndrome chromosome region 16
Aliases:   DKFZp434D0421, MGC189739, MGC44931
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000174374
Entrez:   81554
Chromosome Mapping:   7q11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.940000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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