PrimePCR™ Probe Assay: CORT, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: CORT, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CORT, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029168
Assay Design:   Intron-spanning
Chromosome Location:   1:10510231-10511502question
Amplicon Length:   188
Splice Variants Targeted:   ENST00000377049 ENST00000320498

Gene Information

This gene encodes a neuropeptide that is structurally similar to somatostatin. It binds to all known somatostatin receptors and shares many pharmacological and functional properties with somatostatin including the depression of neuronal activity. However it also has many properties distinct from somatostatin such as induction of slow-wave sleep apparently by antagonism of the excitatory effects of acetylcholine on the cortex reduction of locomotor activity and activation of cation selective currents not responsive to somatostatin. The preproprotein undergoes further processing into multiple mature products. Read-through transcripts exist between this gene and the upstream APITD1 (apoptosis-inducing TAF9-like domain 1) gene as represented in GeneID:100526739. [provided by RefSeq Nov 2010]

Gene Symbol:   CORT
Gene Name:   cortistatin
Aliases:   CST-14, CST-17, CST-29
RefSeq:   NC_000001.10 NT_021937.19
Ensembl:   ENSG00000241563
Entrez:   1325
Chromosome Mapping:   1p36,1p36.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.830000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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