PrimePCR™ Probe Assay: BCAS1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: BCAS1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: BCAS1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029107
Assay Design:   Intron-spanning
Chromosome Location:   20:52561495-52570148question
Amplicon Length:   189
Splice Variants Targeted:   ENST00000448484 ENST00000395961 ENST00000371435 ENST00000422805 ENST00000371440 ENST00000448710 ENST00000434986

Gene Information

This gene resides in a region at 20q13 which is amplified in a variety of tumor types and associated with more aggressive tumor phenotypes. Among the genes identified from this region it was found to be highly expressed in three amplified breast cancer cell lines and in one breast tumor without amplification at 20q13.2. However this gene is not in the common region of maximal amplification and its expression was not detected in the breast cancer cell line MCF7 in which this region is highly amplified. Although not consistently expressed this gene is a candidate oncogene. [provided by RefSeq Jul 2008]

Gene Symbol:   BCAS1
Gene Name:   breast carcinoma amplified sequence 1
Aliases:   AIBC1, NABC1
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000064787
Entrez:   8537
Chromosome Mapping:   20q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.430000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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