PrimePCR™ Probe Assay: SEMA3F, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SEMA3F, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SEMA3F, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029001
Assay Design:   Intron-spanning
Chromosome Location:   3:50214237-50219766question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000414301 ENST00000450338 ENST00000413852 ENST00000002829 ENST00000434342

Gene Information

The semaphorins are a family of proteins that are involved in signaling. All the family members have a secretion signal a 500-amino acid sema domain and 16 conserved cysteine residues (Kolodkin et al. 1993 [PubMed 8269517]). Sequence comparisons have grouped the secreted semaphorins into 3 general classes all of which also have an immunoglobulin domain. The semaphorin III family consisting of human semaphorin III (SEMA3A; MIM 603961) chicken collapsin and mouse semaphorins A D and E all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones (Luo et al. 1993 [PubMed 8402908]) through an interaction with a collapsin response mediator protein of relative molecular mass 62K (CRMP62) (Goshima et al. 1995 [PubMed 7637782]) a putative homolog of an axonal guidance associated UNC33 gene product (MIM 601168). SEMA3F is a secreted member of the semaphorin III family.[supplied by OMIM Mar 2008]

Gene Symbol:   SEMA3F
Gene Name:   sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F
Aliases:   SEMA-IV, SEMA4, SEMAK
RefSeq:   NC_000003.11 NG_009831.1 NT_022517.18
Ensembl:   ENSG00000001617
Entrez:   6405
Chromosome Mapping:   3p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.140000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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