PrimePCR™ Probe Assay: REEP5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028936
Assay Design:   Intron-spanning
Chromosome Location:   5:112238084-112256947question
Amplicon Length:   190
Splice Variants Targeted:   ENST00000379638 ENST00000513339 ENST00000261482 ENST00000545426

Gene Information

Description Not Available

Gene Symbol:   REEP5
Gene Name:   receptor accessory protein 5
Aliases:   C5orf18, D5S346, DP1, MGC70440, TB2, YOP1
RefSeq:   NC_000005.9 NT_034772.6
Ensembl:   ENSG00000129625
Entrez:   7905
Chromosome Mapping:   5q22-q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.760000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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