PrimePCR™ Probe Assay: PFKL, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PFKL, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PFKL, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028823
Assay Design:   Intron-spanning
Chromosome Location:   21:45741739-45742906question
Amplicon Length:   123
Splice Variants Targeted:   ENST00000349048 ENST00000403390 ENST00000534847

Gene Information

Phosphofructokinase (PFK) is a tetrameric enzyme that catalyzes a key step in glycolysis namely the conversion of D-fructose 6-phosphate to D-fructose 16-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits PFK from liver is a homotetramer of L-subunits while PFK from platelets can be composed of any tetrameric combination of M and L subunits. The protein encoded by this gene represents the L subunit. Alternate splicing results in two transcript variants one of which is a candidate for nonsense-mediated decay (NMD). [provided by RefSeq Aug 2008]

Gene Symbol:   PFKL
Gene Name:   phosphofructokinase, liver
Aliases:   DKFZp686G1648, DKFZp686L2097, FLJ30173, FLJ40909, PFK-B
RefSeq:   NC_000021.8 NG_009556.1 NT_011515.12
Ensembl:   ENSG00000141959
Entrez:   5211
Chromosome Mapping:   21q22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.070000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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