PrimePCR™ Probe Assay: PEA15, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PEA15, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PEA15, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028759
Assay Design:   Intron-spanning
Chromosome Location:   1:160181476-160183037question
Amplicon Length:   139
Splice Variants Targeted:   ENST00000360472 ENST00000368076

Gene Information

This gene encodes a death effector domain-containing protein which is a major phosphoprotein in astrocytes and an endogenous substrate for protein kinase C. Studies using knockout mice suggest that this protein may protect astrocytes from TNF-induced apoptosis. This protein is also overexpressed in type 2 diabetes mellitus where it may contribute to insulin resistance in glucose uptake. [provided by RefSeq Sep 2011]

Gene Symbol:   PEA15
Gene Name:   phosphoprotein enriched in astrocytes 15
Aliases:   HMAT1, HUMMAT1H, MAT1, MAT1H, PEA-15, PED
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000162734
Entrez:   8682
Chromosome Mapping:   1q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.000000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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