PrimePCR™ Probe Assay: TTR, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: TTR, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TTR, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028609
Assay Design:   Intron-spanning
Chromosome Location:   18:29171862-29172917question
Amplicon Length:   102
Splice Variants Targeted:   ENST00000237014 ENST00000432547 ENST00000541025

Gene Information

This gene encodes transthyretin one of the three prealbumins including alpha-1-antitrypsin transthyretin and orosomucoid. Transthyretin is a carrier protein; it transports thyroid hormones in the plasma and cerebrospinal fluid and also transports retinol (vitamin A) in the plasma. The protein consists of a tetramer of identical subunits. More than 80 different mutations in this gene have been reported; most mutations are related to amyloid deposition affecting predominantly peripheral nerve and/or the heart and a small portion of the gene mutations is non-amyloidogenic. The diseases caused by mutations include amyloidotic polyneuropathy euthyroid hyperthyroxinaemia amyloidotic vitreous opacities cardiomyopathy oculoleptomeningeal amyloidosis meningocerebrovascular amyloidosis carpal tunnel syndrome etc. [provided by RefSeq Jan 2009]

Gene Symbol:   TTR
Gene Name:   transthyretin
Aliases:   CTS, CTS1, HsT2651, PALB, TBPA
RefSeq:   NC_000018.9 NG_009490.1 NT_010966.14
Ensembl:   ENSG00000118271
Entrez:   7276
Chromosome Mapping:   18q12.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.060000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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