PrimePCR™ Probe Assay: DTNA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: DTNA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DTNA, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028596
Assay Design:   Intron-spanning
Chromosome Location:   18:32395878-32398172question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000315456 ENST00000556176 ENST00000399114 ENST00000348997 ENST00000399113 ENST00000399119 ENST00000283365 ENST00000269190 ENST00000399121 ENST00000444659 ENST00000269191 ENST00000399097

Gene Information

The protein encoded by this gene belongs to the dystrobrevin subfamily of the dystrophin family. This protein is a component of the dystrophin-associated protein complex (DPC) which consists of dystrophin and several integral and peripheral membrane proteins including dystroglycans sarcoglycans syntrophins and alpha- and beta-dystrobrevin. The DPC localizes to the sarcolemma and its disruption is associated with various forms of muscular dystrophy. Mutations in this gene are associated with left ventricular noncompaction with congenital heart defects. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   DTNA
Gene Name:   dystrobrevin, alpha
Aliases:   D18S892E, DRP3, DTN, DTN-A, FLJ96209, LVNC1
RefSeq:   NC_000018.9 NT_010966.14 NG_009201.1
Ensembl:   ENSG00000134769
Entrez:   1837
Chromosome Mapping:   18q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.180000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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