PrimePCR™ Probe Assay: ZMYND8, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ZMYND8, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ZMYND8, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028526
Assay Design:   Intron-spanning
Chromosome Location:   20:45875149-45878087question
Amplicon Length:   140
Splice Variants Targeted:   ENST00000360911 ENST00000311275 ENST00000458360 ENST00000467200 ENST00000262975 ENST00000471951 ENST00000352431 ENST00000461685 ENST00000372023 ENST00000540497 ENST00000536340 ENST00000396281 ENST00000355972 ENST00000446994

Gene Information

The protein encoded by this gene is a receptor for activated C-kinase (RACK) protein. The encoded protein has been shown to bind in vitro to activated protein kinase C beta I. In addition this protein is a cutaneous T-cell lymphoma-associated antigen. Finally the protein contains a bromodomain and two zinc fingers and is thought to be a transcriptional regulator. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   ZMYND8
Gene Name:   zinc finger, MYND-type containing 8
Aliases:   MGC31836, PRKCBP1, PRO2893, RACK7
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000101040
Entrez:   23613
Chromosome Mapping:   20q13.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 35.230000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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