PrimePCR™ Probe Assay: TSPAN32, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: TSPAN32, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TSPAN32, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028478
Assay Design:   Intron-spanning
Chromosome Location:   11:2329998-2334962question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000182290 ENST00000381121 ENST00000451520

Gene Information

This gene which is a member of the tetraspanin superfamily is one of several tumor-suppressing subtransferable fragments located in the imprinted gene domain of chromosome 11p15.5 an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome Wilms tumor rhabdomyosarcoma adrenocortical carcinoma and lung ovarian and breast cancers. This gene is located among several imprinted genes; however this gene as well as the tumor-suppressing subchromosomal transferable fragment 4 escapes imprinting. This gene may play a role in malignancies and diseases that involve this region and it is also involved in hematopoietic cell function. Alternatively spliced transcript variants have been described but their biological validity has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   TSPAN32
Gene Name:   tetraspanin 32
Aliases:   ART1, FLJ17158, FLJ97586, MGC22455, PHEMX, PHMX, TSSC6
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000064201
Entrez:   10077
Chromosome Mapping:   11p15.5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.860000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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