PrimePCR™ Probe Assay: PCDH15, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PCDH15, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PCDH15, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028471
Assay Design:   Intron-spanning
Chromosome Location:   10:55943238-55944950question
Amplicon Length:   143
Splice Variants Targeted:   ENST00000373965 ENST00000395438 ENST00000409834 ENST00000395445 ENST00000395446 ENST00000361849 ENST00000395433 ENST00000373957 ENST00000320301 ENST00000395430 ENST00000373955 ENST00000414778 ENST00000455746 ENST00000395432 ENST00000417177 ENST00000437009

Gene Information

This gene is a member of the cadherin superfamily. Family members encode integral membrane proteins that mediate calcium-dependent cell-cell adhesion. It plays an essential role in maintenance of normal retinal and cochlear function. Mutations in this gene result in hearing loss and Usher Syndrome Type IF (USH1F). Extensive alternative splicing resulting in multiple isoforms has been observed in the mouse ortholog. Similar alternatively spliced transcripts are inferred to occur in human and additional variants are likely to occur. [provided by RefSeq Dec 2008]

Gene Symbol:   PCDH15
Gene Name:   protocadherin-related 15
Aliases:   CDHR15, DFNB23, DKFZp667A1711, USH1F
RefSeq:   NC_000010.10 NT_030059.13 NG_009191.1
Ensembl:   ENSG00000150275
Entrez:   65217
Chromosome Mapping:   10q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.100000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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