PrimePCR™ Probe Assay: IRF5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: IRF5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: IRF5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028441
Assay Design:   Intron-spanning
Chromosome Location:   7:128582302-128586040question
Amplicon Length:   141
Splice Variants Targeted:   ENST00000489702 ENST00000357234 ENST00000477535 ENST00000479582 ENST00000464557 ENST00000402030 ENST00000473745 ENST00000249375 ENST00000412326 ENST00000430204 ENST00000453794

Gene Information

This gene encodes a member of the interferon regulatory factor (IRF) family a group of transcription factors with diverse roles including virus-mediated activation of interferon and modulation of cell growth differentiation apoptosis and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Multiple transcript variants encoding different isoforms have been found for this gene and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment. [provided by RefSeq Mar 2010]

Gene Symbol:   IRF5
Gene Name:   interferon regulatory factor 5
Aliases:   SLEB10
RefSeq:   NC_000007.13 NT_007933.15 NG_012306.1
Ensembl:   ENSG00000128604
Entrez:   3663
Chromosome Mapping:   7q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.120000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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