PrimePCR™ Probe Assay: NPAS2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028149
Assay Design:   Intron-spanning
Chromosome Location:   2:101549377-101554263question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000335681 ENST00000427413 ENST00000448812 ENST00000451740 ENST00000542504

Gene Information

The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH)-PAS family of transcription factors. A similar mouse protein may play a regulatory role in the acquisition of specific types of memory. It also may function as a part of a molecular clock operative in the mammalian forebrain. [provided by RefSeq Jul 2008]

Gene Symbol:   NPAS2
Gene Name:   neuronal PAS domain protein 2
Aliases:   FLJ23138, MGC71151, MOP4, PASD4, bHLHe9
RefSeq:   NC_000002.11 NG_023259.1 NT_022171.15
Ensembl:   ENSG00000170485
Entrez:   4862
UniGene:   Hs.156832
Chromosome Mapping:   2q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.860000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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