PrimePCR™ Probe Assay: SCD5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SCD5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SCD5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028087
Assay Design:   Intron-spanning
Chromosome Location:   4:83626440-83719487question
Amplicon Length:   126
Splice Variants Targeted:   ENST00000319540 ENST00000273908 ENST00000282709

Gene Information

Stearoyl-CoA desaturase (SCD; EC 1.14.99.5) is an integral membrane protein of the endoplasmic reticulum that catalyzes the formation of monounsaturated fatty acids from saturated fatty acids. SCD may be a key regulator of energy metabolism with a role in obesity and dislipidemia. Four SCD isoforms Scd1 through Scd4 have been identified in mouse. In contrast only 2 SCD isoforms SCD1 (MIM 604031) and SCD5 have been identified in human. SCD1 shares about 85% amino acid identity with all 4 mouse SCD isoforms as well as with rat Scd1 and Scd2. In contrast SCD5 shares limited homology with the rodent SCDs and appears to be unique to primates (Wang et al. 2005 [PubMed 15907797]).[supplied by OMIM Mar 2008]

Gene Symbol:   SCD5
Gene Name:   stearoyl-CoA desaturase 5
Aliases:   ACOD4, FADS4, FLJ21032, HSCD5, SCD2, SCD4
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000145284
Entrez:   79966
Chromosome Mapping:   4q21.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.010000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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