PrimePCR™ Probe Assay: MAP2K1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MAP2K1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MAP2K1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028077
Assay Design:   Intron-spanning
Chromosome Location:   15:66774149-66777409question
Amplicon Length:   121
Splice Variants Targeted:   ENST00000307102 ENST00000425818

Gene Information

The protein encoded by this gene is a member of the dual specificity protein kinase family which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases also known as extracellular signal-regulated kinases (ERKs) act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway this kinase is involved in many cellular processes such as proliferation differentiation transcription regulation and development. [provided by RefSeq Jul 2008]

Gene Symbol:   MAP2K1
Gene Name:   mitogen-activated protein kinase kinase 1
Aliases:   MAPKK1, MEK1, MKK1, PRKMK1
RefSeq:   NC_000015.9 NG_008305.1 NT_010194.17
Ensembl:   ENSG00000169032
Entrez:   5604
Chromosome Mapping:   15q22.1-q22.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.120000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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