PrimePCR™ Probe Assay: DIRAS3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: DIRAS3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DIRAS3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028071
Assay Design:   Intron-spanning
Chromosome Location:   1:68512915-68516254question
Amplicon Length:   125
Splice Variants Targeted:   ENST00000370981 ENST00000395201

Gene Information

This gene is a member of the ras superfamily and is expressed in normal ovarian and breast epithelial cells but not in ovarian and breast cancers. It is an imprinted gene with monoallelic expression of the paternal allele which is associated with growth suppression. Thus this gene appears to be a putative tumor suppressor gene whose function is abrogated in ovarian and breast cancers. [provided by RefSeq Oct 2010]

Gene Symbol:   DIRAS3
Gene Name:   DIRAS family, GTP-binding RAS-like 3
Aliases:   ARHI, NOEY2
RefSeq:   NC_000001.10 NG_011753.1 NT_032977.9
Ensembl:   ENSG00000162595
Entrez:   9077
Chromosome Mapping:   1p31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 34.700000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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