PrimePCR™ Probe Assay: ECH1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ECH1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ECH1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028047
Assay Design:   Intron-spanning
Chromosome Location:   19:39308103-39321784question
Amplicon Length:   150
Splice Variants Targeted:   ENST00000221418

Gene Information

This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species particularly within a conserved domain characteristic of these proteins. The encoded protein which contains a C-terminal peroxisomal targeting sequence localizes to the peroxisome. The rat ortholog which localizes to the matrix of both the peroxisome and mitochondria can isomerize 3-trans5-cis-dienoyl-CoA to 2-trans4-trans-dienoyl-CoA indicating that it is a delta35-delta24-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. [provided by RefSeq Jul 2008]

Gene Symbol:   ECH1
Gene Name:   enoyl CoA hydratase 1, peroxisomal
Aliases:   HPXEL
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000104823
Entrez:   1891
Chromosome Mapping:   19q13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 34.850000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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