PrimePCR™ Probe Assay: CDK5RAP3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CDK5RAP3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CDK5RAP3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027902
Assay Design:   Intron-spanning
Chromosome Location:   17:46051371-46052600question
Amplicon Length:   122
Splice Variants Targeted:   ENST00000536708 ENST00000338399

Gene Information

Neuronal CDC2-like kinase which is involved in the regulation of neuronal differentiation is composed of a catalytic subunit CDK5 and an activating subunit p25NCK5A. The protein encoded by this gene binds to p25NCK5A and therefore may be involved in neuronal differentiation. The encoded protein which may be a substrate of neuronal CDC2-like kinase has also been found in vascular endothelial cells where it mediates cell proliferation. [provided by RefSeq Jul 2008]

Gene Symbol:   CDK5RAP3
Gene Name:   CDK5 regulatory subunit associated protein 3
Aliases:   C53, HSF-27, IC53, LZAP, MST016, OK/SW-cl.114
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000108465
Entrez:   80279
Chromosome Mapping:   17q21.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996000
y-intercept 35.660000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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